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KMID : 0603820090150040287
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Journal of Experimental & Biomedical Science
2009 Volume.15 No. 4 p.287 ~ p.293
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Involvement of Phospholipase D in Norepinephrine Uptake in PC12 Cells
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Rhee Jong-Joo
Oh Sae-Ock
Kim Young-Rae
Park Jong-Il
Park Seung-Kiel
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Abstract
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Phospholipase D (PLD) is an enzyme hydrolyzing phosphatidylcholine to phosphatidic acid (PA) and choline. We investigated the involvement of PLD1 in the uptake of norepinephrine (NE) in PC12 cells, pheochromocytoma cells. NE uptake was specific in PC12 cells because nomifensine, a specific blocker of NE transporter, blocked NE uptake. Inhibition of PLD function in PC12 cells by the treatment of butanol suppressed the NE uptake. In contrast, overexpression of PLD1 in PC12 cells increased NE uptake efficiently. These results suggest that PLD activity is involved in NE uptake. We explored the action mechanism of PLD in NE uptake. PA phosphatase inhibitor, propranolol, blocks the formation of PKC activator diacylglycerol from PA. Propranolol treatment to PC12 cells blocked dramatically the uptake of NE. Specific PKC inhibitors, GF109203X and Ro31-8220, blocked NE uptake. Taken together, we suggest for the first time that PLD1 activity is involved in NE uptake via the activation of PKC.
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KEYWORD
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Norepinephrine, Phosphatidic acid, Phospholipase D, Protein kinase C, Transporter, Uptake
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